JPK Nanotracker™

承上一篇,大家可以到以下兩個網址連結到JPK Nanotracker的詳細介紹:
http://www.jpk.com/opt-tweezers-3d-particle-tracking.29.html


Optical tweezers enable trapping and manipulation of nanoparticles, microparticles, and biological species in fluid media. Now, JPK's unique Nanotracker™ system extends this technology to enable measurement of interaction forces with sub picoNewton sensitivity. In addition, particles are simultaneously tracked in 3-D to quantify dynamics, viscosity, diffusion and host of other processes.
For the first time, dual beam force-sensing optical tweezers seamlessly integrate on inverted optical microscopes combining advanced optical and confocal techniques including single molecule fluorescence in a small footprint, easy to use system.
Our unique tweezers technology (also known as a Photonic Force Microscope) enables quantification of molecular, cellular and micro-rheological processes. Applications include molecular motor mechanics, binding/elasticity of DNA and proteins, cell membrane dynamics and particle uptake. JPK's Nanotracker™ is set to revolutionize research in biophysics, biochemistry, drug discovery, toxicology and many other fields.

http://www.jpk.com/nanotracker-tm.387.html


With the NanoTracker™, the user can trap and track particles from several µm down to 30nm with the ability to control, manipulate and observe samples from vesicles to whole cells in real time with nanometer precision.
NanoTracker™ technology provides precisely quantifiable and reproducible measurements of particle/ cell interactions. The system delivers precise information about single molecule mechanics and may also be used to determine mechanical characteristics such as adhesion, elasticity or stiffness on single molecules.

NanoTracker™ setup with syringe pumpson Zeiss Axio Observer base

Key features
Real-time data - essential for living cell studies
Optical Tweezers meets 3D particle tracking
3D real space tracking information with nanometer precision
Use of biochemically modified nanoparticles
No perturbation of the biological system
Reproducible and calibrated data
Quantitative results from mechanical properties to diffusion

Quantifying cell-virus interactions using the NanoTracker™ optical tweezers system

這是Alexander Rohrbach license 給JPK的PFM的研究成果,給大家參考一下.希望我們的Optical Tweezers也能一直deliver這樣的研究成果.

Figure 1: The NanoTracker™ optical tweezers platform.


Figure 3: DIC image of a CHO cell with a membrane tether pulled by an optically trapped microsphere..

The movie in Figure 3 shows such a tether being pulled out from the membrane of a CHO cell. After an interaction time of two seconds, the virus-coated microsphere was retracted with a speed of 5 µm/s by moving the piezo stage. In figure 4, the same experiment was performed at a higher retraction speed. The virus-coated particles were pushed into the cells with a force of about 20 pN (blue area in the graph). The viruses were let to interact with the cell (white area) and then retracted at 0.5 µm/s. From the stepwise unbinding seen in this phase we can deduce that multiple bonds had been formed.



Figure 4: Measuring forces between cells and specifically coated microspheres. Approach and retract phases are shown. The retract phase shows a bond rupture in several steps.

完整文章請點下面連結:

http://www.netdyalog.com/news/jpk/33/020.asp?453928-496911-468926-045616